Fig. 4
Characterization of Dv v-ATPase C RNAi expression in maize transgenic events using RNase If -qPCR. The total RNAs isolated from the leaf tissues of distinct transgenic maize T0 events carrying v-ATPase C RNAi. The RNAi transcripts were analyzed to quantify dsRNAs (R95 RTL) and ssRNAs (70 RTL), respectively. R70 RTL was assayed as control for RNase If treatment. The R70 RTL indicated effective RNase If treatment (data not shown). The RTL were normalized to endogenous gene (TIP41). The dsRNA and ssRNA RTL are represented as dark and light gray respectively