Table 1 Expression of β-glucosidase and autolysis efficiency of various strains in 250 mL Erlenmeyer flasks
From: Light induced expression of β-glucosidase in Escherichia coli with autolysis of cell
strains and plasmids | inducer | β-glucosidases activity (U/mL) | autolysis efficiency |
|---|---|---|---|
BL21(DE3) + pET22b–pD-bgl | Light | 33.22 ± 2.01 | |
BL21(DE3) + pET22b-T7-bgl | IPTG | 34.02 ± 1.96 | |
BL21(DE3)pLysS + pET22b–pD-bgl-T7-SRRz | Light + lactose | 24.14 ± 2.14 | 84.53 ± 6.79% |
Light + IPTG | 25.53 ± 1.64 | 94.58 ± 3.10% | |
BL21(DE3)pLysS + pET22b–pD-bgl-CBM-T7-SRRz | Light + lactose | 21.45 ± 2.59 | 85.34 ± 5.31% |