Table 1 Optimization of P0 and P1 virus generation
From: A new single-step protocol for rapid baculovirus-driven protein production in insect cells
Construct | DNA:PEI | Cell count × 106 | Viability at harvest (%) | Diameter at harvest (μm) |
Cells before transfection* | 0.8 | 91.5 | 21.2 | |
#1 | 1:2 | 1.0 | 91.1 | 23.1 |
#1 | 1:4 | 0.7 | 75.2 | 22.1 |
#1 | 1:6 | 0.5 | 59.4 | 21.4 |
#2 | 1:2 | 1.0 | 89.0 | 25.3 |
#2 | 1:4 | 0.7 | 81.0 | 24.2 |
#2 | 1:6 | 0.4 | 70.0 | 22.0 |
Construct | P0 dilution | Cell count × 106 | Viability at harvest (%) | Diameter at harvest (μm) |
#1 | 2500 | 0.6 | 74 | 23.7 |
#1 | 5000 | 1.0 | 78.4 | 24.6 |
#2 | 2500 | 0.4 | 62.4 | 24.1 |
#2 | 5000 | 0.9 | 74.1 | 24.9 |