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Table 1 Optimization of P0 and P1 virus generation

From: A new single-step protocol for rapid baculovirus-driven protein production in insect cells

Construct DNA:PEI Cell count × 106 Viability at harvest (%) Diameter at harvest (μm)
Cells before transfection*   0.8 91.5 21.2
#1 1:2 1.0 91.1 23.1
#1 1:4 0.7 75.2 22.1
#1 1:6 0.5 59.4 21.4
#2 1:2 1.0 89.0 25.3
#2 1:4 0.7 81.0 24.2
#2 1:6 0.4 70.0 22.0
Construct P0 dilution Cell count × 106 Viability at harvest (%) Diameter at harvest (μm)
#1 2500 0.6 74 23.7
#1 5000 1.0 78.4 24.6
#2 2500 0.4 62.4 24.1
#2 5000 0.9 74.1 24.9
  1. Sf9 cell parameters for different DNA:PEI ratio and different P0 dilutions used for P1 generation; * Sf9 cells were split 2–3 h prior to transfection or infection; data were recorded immediately before transfection or infection