Fig. 1
From: A new single-step protocol for rapid baculovirus-driven protein production in insect cells
Comparison of different baculovirus expression strategies using Tn7 transposon mediated gene integration. The recombinant EMBacY bacmid carries the integrated gene of interest GOI and YFP for visualization. a Conventional Bac-to-Bac procedure with transfection of adherent cells to generate P0 baculovirus followed by one or more subsequent virus amplification steps P1and P2. b Titerless infected-cells preservation TIPS protocol. Insect cells infected with P0 virus are frozen prior to cell lysis and used for protein expression in screening and production. c Single-step procedure presented in this study. P0 virus is directly used for expression screening and production