Fig. 2

Construction and expression of the recombinant LAH3-HBc gene. Design (a) and cartoon (b) of the LAH3-HBc dimer. Individual HBc monomers are coloured in green and orange. First MIR contains the LAH domain (represented as random pink spheres) while the second MIR contains lysine linker (blue spheres). The model was created using PyMOL version 1.7rc1. The tandem core dimer was based on the structure from PDB-1QGT, with the linker added in black. c LAH3-HBc fermentation profile. Dissolved oxygen tension (DOT) was maintained above 30% through agitation control (N) between 400 to 1000 rpm. Biomass was monitored post-induction by wet cell weight (WCW) analysis