Fig. 3
From: A split ubiquitin system to reveal topology and released peptides of membrane proteins
Subcellular localization and performance of SUT on a membrane protein with N terminal released peptide. a-b Confocal micrographs of WKS1.1-GFP (a) or GFP-WKS1.1 (b) fusion proteins in tabaco epidermal cells. Bar stands for 25Â ÎĽm. Green: GFP fusion proteins; red: chloroplast autofluorescence; bright light: cell shape; and overlay of the three panels. c Detection of WKS1.1-GFP or GFP-WKS1.1 fusion proteins with anti GFP antibody. N, GFP-WKS1.1; C, WKS1.1-GFP; GFP, empty vector. Duplicate experiments were shown with N and C samples. d Growth of yeast cells with WKS1.1 and control plasmids in SUT system. e Growth of yeast cells with WKS1.1 in the SUT system