Fig. 1

Subcellular localization and yeast growth of a typical nucleocytoplasmic protein. a-b Confocal micrographs of GUS-GFP (a) or GFP-GUS (b) fusion proteins in tabaco epidermal cells. The panels from left to right stand for: GFP (green), chloroplast auto-fluorescence (red), cell shape under bright light and overlay of the three panels. Bar stands for 25 μm. GFP, green; chloroplast autofluorescence, red (Chl); cell shape, bright light; and overlay of the three panels. c Yeast growth of GUS in SUT system. M, plasma membrane; Cyt, cytoplasm; Cub, C terminal ubiquitin; Nub^WT, wild type N terminal ubiquitin; Nub^G, low affinity N terminal ubiquitin with Ile13Gly mutation