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Table 3 Product yield during semi-anaerobic fermentation and xylose isomerase activity performed in vitro with crude extract

From: Conversion of an inactive xylose isomerase into a functional enzyme by co-expression of GroEL-GroES chaperonins in Saccharomyces cerevisiae

Strain

Xylitol Yield

(g xylitol/g sugars)

Glycerol Yield

(g glycerol/g sugars)

Acetic Acid Yield

(g acetic acid/g sugars)

Ethanol Yield

(g ethanol/g sugars)

Activity (U/mL)

BTY28

(pRS426)

0.000

0.000

0.000

0.161

ND

BTY29

(XIOrp)

0.012

0.043

0.012

0.377

0.073 ± 0.011

BTY30

(XI2.0)

0.005

0.013

0.001

0.198

0.023 ± 0.008

BTY32

(Gro; pRS426)

0.011

0.016

0.000

0.205

ND

BTY33

(Gro;XIOrp)

0.009

0.041

0.010

0.444

0.087 ± 0.006

BTY34

(Gro;XI2.0)

0.014

0.053

0.007

0.441

0.095 ± 0.008