Table 2 Summary of in silico analyses for assessment of allergenic potential of the chimeric cry proteins
Cry Proteins | Full length FASTA search | 80mer sliding window search | NCBI DELTA-BLAST (% identity& E-value) | Algpred 1.0 | AllerTop 1.0 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Allergen online 17.0 (% identity& E-value) | SDAP (% identity) | Allergen online 17.0 (% identity) | SDAP (% identity) | ||||||||
Cry1Aabc | No matches with E score < 1.0 | 9.02% | Gly m Bd2 8 K, (BAB21619) | No matches with >35% | 28.75% | Asp f 13 (P28296) | No matches with E score < 0.01 | Non –Allergen | Probably Non - Allergen | ||
5.80% | Ana c 2 (BAA21849) | 26.25% | Der f 1.0102 (2428875) | ||||||||
5.80% | Tri a gliadin (AAA34285) | 27.50% | Alt a 2 (AAD00097) | ||||||||
Cry1AcF | 23.7% | Gamma-Gliadin (170738) E - 0.49 | 8.99% | Gly m Bd2 8 K (BAB21619) | No matches with >35% | 27.50% | Hev b 1 (P15252) | 17% | Pectate lyase/Amb allergen (EEU00825.1) E – 9e-07 | Non –Allergen | Probably Non - Allergen |
5.78% | Tri a gliadin (AAA34285) | 27.50% | Alt a 2 (AAD00097) | 17% | Pectate lyase/Amb allergen (ACX76150.1) E – 2e-06 | ||||||
5.78% | Eur m 3 (O97370) | 26.25% | Lig v 1 (O82015) | 15% | Fibronectin type III domain protein (ACT03889.1) E – 6e-04 | ||||||
Cry1AbBaBa | No matches with E score < 1.0 | 6.28% | Ani s11.0101(BAJ78220) | No matches with >35% | 28.75% | Pis v 3.0101 (EF116865) | No matches with E score < 0.01 | Non –Allergen | Probably Non - Allergen | ||
4.71% | Lig v 1(O82015) | 26.25% | Pet c PR10 (CAA67246) | ||||||||
26.25% | Lig v 1 (O82015) | ||||||||||
Cry1BaBaAb | 26.8% | Gamma-Gliadin precursor (1063270) E − 0.65 | 5.58% | Mala s 1 (Q01940) | No matches with >35% | 32.50% | Mala s 1 (Q01940) | 14% | Pectate lyase/Amb allergen (ABN54148.1) E – 8e-08 | Non –Allergen | Probably Non - Allergen |
24.0% | Der f Mal f 6 allergen (37958141) E − 0.96 | 4.50% | Asp f 17 (CAA12162) | 27.50% | Asp f 17 (CAA12162) | 14% | Pectate lyase/Amb allergen (EEU00825.1) E – 7e-08 | ||||
4.19% | Pis v 4.0101 (EF470980) | 26.25% | Asp f 10 (CAA59419) | ||||||||
Cry1AbBaAb | No matches with E score < 1.0 | 5.77% | Mala s 1 (Q01940) | No matches with >35% | 32.50% | Mala s 1 (Q01940) | 14% | Pectate lyase/Amb allergen (EEU00825.1) E – 9e-08 | Non –Allergen | Probably Non - Allergen | |
4.81% | Lig v 1 (O82015) | 27.50% | Asp f 17 (CAA12162) | 14% | Pectate lyase/Amb allergen (ABN54148.1) E – 1e-07 | ||||||
4.81% | Asp f 17 (CAA12162) | 26.25% | Asp f 10 (CAA59419) | ||||||||
Cry1AcJAc | No matches with E score < 1.0 | 7.62% | Gly m 6.0501 (Q7GC77) | No matches with >35% | 32.50% | Api m 3.0101 (NP_00101337) | No matches with E score < 0.01 | Non –Allergen | Probably Non - Allergen | ||
7.28% | Api m 3.0101 (NP_00101337) | 30.0% | Dol m 1.0101 (Q06478) | ||||||||
5.79% | Eur m 3 (O97370) | 28.75% | Gly m 1 (AAB09252) | ||||||||
Cry1AaIa5Ia5 | No matches with E score < 1.0 | 8.41% | Per a 3.0101 (Q25641) | No matches with >35% | 26.5% | Lig v 1 (O82015) | No matches with E score < 0.01 | Non –Allergen | Probably Non - Allergen | ||
6.23% | Sol i 1.0101 (AAT95008) | 26.5% | Pol f 5 (P35780) | ||||||||
5.51% | Asp f 4 (O60024) | 25% | Ana c 1 (AAK54835) | ||||||||
Cry1AaB | No matches with E score < 1.0 | 6.21% | Ani s 11.0101 (BAJ78220) | No matches with >35% | 27.50% | Alta2 (AAD00097) | No matches with E score < 0.01 | Non –Allergen | Probably Non - Allergen | ||
5. | Car p papain (AAB02650) | 26.50% | Ligv1 (O82015) | ||||||||
4.94% | Alt a 2 (AAD00097) | 25% | Pen c 24 (AAR17475) | ||||||||