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Table 2 Summary of in silico analyses for assessment of allergenic potential of the chimeric cry proteins

From: Comprehensive in silico allergenicity assessment of novel protein engineered chimeric Cry proteins for safe deployment in crops

Cry Proteins Full length FASTA search 80mer sliding window search NCBI DELTA-BLAST (% identity& E-value) Algpred 1.0 AllerTop 1.0
Allergen online 17.0 (% identity& E-value) SDAP (% identity) Allergen online 17.0 (% identity) SDAP (% identity)
Cry1Aabc No matches with E score < 1.0 9.02% Gly m Bd2 8 K, (BAB21619) No matches with >35% 28.75% Asp f 13 (P28296) No matches with E score < 0.01 Non –Allergen Probably Non - Allergen
5.80% Ana c 2 (BAA21849) 26.25% Der f 1.0102 (2428875)
5.80% Tri a gliadin (AAA34285) 27.50% Alt a 2 (AAD00097)
Cry1AcF 23.7% Gamma-Gliadin (170738) E - 0.49 8.99% Gly m Bd2 8 K (BAB21619) No matches with >35% 27.50% Hev b 1 (P15252) 17% Pectate lyase/Amb allergen
(EEU00825.1)
E – 9e-07
Non –Allergen Probably Non - Allergen
5.78% Tri a gliadin (AAA34285) 27.50% Alt a 2 (AAD00097) 17% Pectate lyase/Amb allergen
(ACX76150.1)
E – 2e-06
5.78% Eur m 3 (O97370) 26.25% Lig v 1 (O82015) 15% Fibronectin type III domain protein
(ACT03889.1)
E – 6e-04
Cry1AbBaBa No matches with E score < 1.0 6.28% Ani s11.0101(BAJ78220) No matches with >35% 28.75% Pis v 3.0101 (EF116865) No matches with E score < 0.01 Non –Allergen Probably Non - Allergen
4.71% Lig v 1(O82015) 26.25% Pet c PR10 (CAA67246)
26.25% Lig v 1 (O82015)
Cry1BaBaAb 26.8% Gamma-Gliadin precursor (1063270) E − 0.65 5.58% Mala s 1 (Q01940) No matches with >35% 32.50% Mala s 1 (Q01940) 14% Pectate lyase/Amb allergen
(ABN54148.1)
E – 8e-08
Non –Allergen Probably Non - Allergen
24.0% Der f Mal f 6 allergen (37958141) E − 0.96 4.50% Asp f 17 (CAA12162) 27.50% Asp f 17 (CAA12162) 14% Pectate lyase/Amb allergen
(EEU00825.1)
E – 7e-08
4.19% Pis v 4.0101 (EF470980) 26.25% Asp f 10 (CAA59419)
Cry1AbBaAb No matches with E score < 1.0 5.77% Mala s 1 (Q01940) No matches with >35% 32.50% Mala s 1 (Q01940) 14% Pectate lyase/Amb allergen
(EEU00825.1)
E – 9e-08
Non –Allergen Probably Non - Allergen
4.81% Lig v 1 (O82015) 27.50% Asp f 17 (CAA12162) 14% Pectate lyase/Amb allergen
(ABN54148.1)
E – 1e-07
4.81% Asp f 17 (CAA12162) 26.25% Asp f 10 (CAA59419)
Cry1AcJAc No matches with E score < 1.0 7.62% Gly m 6.0501 (Q7GC77) No matches with >35% 32.50% Api m 3.0101 (NP_00101337) No matches with E score < 0.01 Non –Allergen Probably Non - Allergen
7.28% Api m 3.0101 (NP_00101337) 30.0% Dol m 1.0101 (Q06478)
5.79% Eur m 3 (O97370) 28.75% Gly m 1 (AAB09252)
Cry1AaIa5Ia5 No matches with E score < 1.0 8.41% Per a 3.0101 (Q25641) No matches with >35% 26.5% Lig v 1 (O82015) No matches with E score < 0.01 Non –Allergen Probably Non - Allergen
6.23% Sol i 1.0101 (AAT95008) 26.5% Pol f 5 (P35780)
5.51% Asp f 4 (O60024) 25% Ana c 1 (AAK54835)
Cry1AaB No matches with E score < 1.0 6.21% Ani s 11.0101 (BAJ78220) No matches with >35% 27.50% Alta2 (AAD00097) No matches with E score < 0.01 Non –Allergen Probably Non - Allergen
5. Car p papain (AAB02650) 26.50% Ligv1 (O82015)
4.94% Alt a 2 (AAD00097) 25% Pen c 24 (AAR17475)