Fig. 8From: Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene – methyltetrazine reactionsConjugation reaction of rFab’-MTZ and hFasLECD-TCO analyzed by high-performance size-exclusion chromatography. Panels: a, rFab-MTZ alone; b–e, reaction mixtures (b, 1.0; c, 2.0; d, 3.0 and e 5.0 M excess amounts of rFab’-MTZ were reacted with hFasLECD-TCO). Retention time of each peak is shownBack to article page