Fig. 3

Enzymatic activity and stability of recombinant PFA at different temperatures (a and c) and pH values (b and d). a The reaction was carried out in acetate buffer at pH 5.5. b The reaction was carried out in acetate buffer at pH 3-7, and in phosphate buffer at pH 7-12.5. c Plant-made PFA was extracted with reducing buffer, diluted 1/20 in acetate buffer pH 5.5 and incubated at different temperatures. d Plant-made PFA was extracted with reducing buffer, diluted 1/20 in buffers with different pH values and incubated at room temperature. The enzymatic assay was carried out at 95 °C