Fig. 4
From: Development of plant-produced protein body vaccine candidates for bluetongue virus
Purification of PBs. a Western blot analysis of crude Zera®-VP2ep (i) and Zera®-VP2 (ii) extracted protein using α-VP2R as primary antibody. White arrows indicate the respective proteins with the black arrow showing an oligomerised fusion of VP2ep. In both cases the negative control (−) was plant material infiltrated with infiltration medium only that was extracted using the same method. Lanes M indicate the molecular weight marker in kDa. b α-VP2R dot blots of (i) the negative control, (ii) Zera®-VP2ep and (iii) Zera®-VP2 purified using a 42% sucrose cushion