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Table 2 Relative enzymatic activity of pdc and adhB in four different engineered strains and standard error of the mean value (in brackets) for at least two independent measurements

From: Fermentation of lactose to ethanol in cheese whey permeate and concentrated permeate by engineered Escherichia coli

Engineered strain

Relative pdc activitya

Relative adhB activitya

MG1655-pL13

0.22 (0.005)

0.23 (0.124)

ML308-pL13

1 (-)

1 (-)

W-pL13

0.156 (0.012)

0.17 (0.019)

W-pLOI297

1.26 (0.325)

2.32 (0.831)

  1. aAll the reported activities for both pdc and adhB are normalized by the ones of ML308-pL13, measured in the same experiment. For this reason, the activity values of ML308-pL13 are always 1 and no standard error can be computed. Wild type strains were also assayed as controls and they showed no detectable activity for both enzymes (data not shown)
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BMC Biotechnology

ISSN: 1472-6750

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