Fig. 7
Inhibitory effect of anti-ST8SiaII-IB and anti-ST8SiaIV-IB on metastasis of rhabdyomasarcoma cells after 4 weeks of tumor cell injection in mice. a Detection of tumor cells by luminescence. 106 rhabdomyosarcoma cells in 100 μl PBS expressing anti-ST8SiaII-IB and anti-ST8SiaIV-IB or anti-NCAM-IB or as negative control tumor cells stable transfected with the empty vector were injected intraperitoneally into 3 C57BL/6 J RAG-2 mice at a time. Luminiscence was determined at week 4 using in vivo imaging systems (IVIS). Shown are the results as luminiscence signals (p/sec/cm2/sr). ROI: region of interest. The red circles shows the metastic tumor cells in the region of lung and liver. b Mean values of each group of mice with corresponding standard deviation. Tumor growth was tracked every week over a period of 6 weeks. Complete tumor tracking is shown in supplement data