Distinctive molecular and biochemical characteristics of a glycoside hydrolase family 20 β-N-acetylglucosaminidase and salt tolerance

Table 4 Effects of metal ions and chemical reagents on the activity of purified rHJ5Nag

Reagent	Relative activity (%)^a		Reagent	Relative activity (%)^a
Reagent	1.0 mM	10.0 mM	Reagent	1.0 mM	10.0 mM
None	100.0 ± 0.4	100.0 ± 2.7	MnSO₄	100.9 ± 2.6	99.6 ± 2.4
FeCl₃	117.4 ± 1.8	103.1 ± 0.0	ZnSO₄	96.8 ± 3.1	84.5 ± 9.9
MgSO₄	113.8 ± 5.8	99.3 ± 3.4	AgNO₃	0.0	0.0
CaCl₂	108.7 ± 5.4	96.6 ± 3.5	HgCl₂	0.0	0.0
KCl	105.4 ± 4.8	101.4 ± 1.3	Tween 80	131.5 ± 1.7^b	108.1 ± 2.1^c
CoCl₂	103.7 ± 3.1	100.4 ± 1.7	EDTA	106.7 ± 4.5	99.5 ± 2.1
NiSO₄	101.7 ± 8.7	95.0 ± 0.7	β-Mercaptoethanol	106.1 ± 1.2	100.1 ± 0.6
CuSO₄	101.4 ± 4.8	88.6 ± 5.5	Triton X-100	100.5 ± 4.7^b	93.9 ± 5.2^c
Pb(CH₃COO)₂	101.0 ± 5.8	106.7 ± 3.7	SDS	0.0	0.0

^aValues represent the means ± SD (n = 3) relative to the untreated control sample
^bFinal concentration: 0.5% (w/v)
^cFinal concentration: 1.0% (w/v)

ISSN: 1472-6750