Improved n-butanol production via co-expression of membrane-targeted tilapia metallothionein and the clostridial metabolic pathway in Escherichia coli

Table 4 KEGG biological pathways for significantly expressed genes ^a

Groups	Gene	Expression difference^b	Description
Oxidative phosphorylation	nuoI	-1.88 ↓	NADH:ubiquinone oxidoreductase, chain I
	nuoG	-1.71 ↓	NADH:ubiquinone oxidoreductase, chain G
	nuoF	-1.49 ↓	NADH:ubiquinone oxidoreductase, chain F
	nuoC	-1.35 ↓	NADH:ubiquinone oxidoreductase, fused CD subunit
Fructose and mannose metabolism	fucA	2.26 ↑	L-fuculose-1-phosphate aldolase
	srlE	1.63 ↑	glucitol/sorbitol-specific enzyme IIB component of PTS
	srlA	2.78 ↑	glucitol/sorbitol-specific enzyme IIC component of PTS
Ribosome	rplA	-1.32 ↓	50S ribosomal subunit protein L1
	rpsF	-1.42 ↓	30S ribosomal subunit protein S6
	rpmF	-2.22 ↓	50S ribosomal subunit protein L32
	rplI	-1.34 ↓	50S ribosomal subunit protein L9
Glycolysis/Gluconeogenesis	pfkB	-1.75 ↓	6-phosphofructokinase II
Glycolysis/Gluconeogenesis	pgm	-1.44 ↓	phosphoglucomutase
Nicotinate and nicotinamide metabolism	yjjG	1.24 ↑	dUMP phosphatase
Nicotinate and nicotinamide metabolism	pntA	-1.45 ↓	pyridine nucleotide transhydrogenase, alpha subunit

^aKEGG pathway annotation enrichment analysis for E. coli str. K12 substr. W3110 (Org code : ecj)
^bGenes that changed by FPKM > 0.3 and ≧ 2-fold differences between BUT3-DE vs. BUT1-DE
↑Represents up-regulated genes; ↓ represents down-regulated genes

ISSN: 1472-6750