Fig. 2
From: Construction of a novel anaerobic pathway in Escherichia coli for propionate production
Propionate and other fermentation products of engineered strains with sbm operon under different promoters. Strains in glass screw-cap tubes with NBS (20 g/L glucose) media were cultivated aerobically in the dark at 37 °C, until the cultures reached at an OD600 of 0.5. Then cultures were added with appropriate concentrations of IPTG and cultivated anaerobically in dark for 72 h. All experiments were performed in triplicate