Table 2 FVIII purification sheet
# | Name | Resin | Volume, ml | Protein, mg/mla | FVIII:C, IU/ml | Specific activity, IU/mg | Total FVIII:C, kIU | Stage yield | Overall yield |
|---|---|---|---|---|---|---|---|---|---|
0 | Clarified medium | - | 320 | -- | 36.3 | - | 11.6 | - | - |
1 | Multi-mode anion exchange | Capto MMC | 17 | 0.233 | 496.9 | 2133 | 8.45 | 73% | 73% |
2 | Cation exchange | SP Sepharose | 10 | 0.271 | 538.1 | 1986 | 5.38 | 64% | 46% |
3 | Affinity | VIII Select | 11 | 0.046 | 382.0 | 8304 | 4.20 | 78% | 36% |
4 + 5 | Anion exchange + size exclusion | Capto Q + Superdex 75 | 10 | 0.026 | 284.1 | 10925 | 2.84 | 68% | 24% |