|
#
|
Name
|
Resin
|
Volume, ml
|
Protein, mg/mla
|
FVIII:C, IU/ml
|
Specific activity, IU/mg
|
Total FVIII:C, kIU
|
Stage yield
|
Overall yield
|
|---|
|
0
|
Clarified medium
|
-
|
320
|
--
|
36.3
|
-
|
11.6
|
-
|
-
|
|
1
|
Multi-mode anion exchange
|
Capto MMC
|
17
|
0.233
|
496.9
|
2133
|
8.45
|
73%
|
73%
|
|
2
|
Cation exchange
|
SP Sepharose
|
10
|
0.271
|
538.1
|
1986
|
5.38
|
64%
|
46%
|
|
3
|
Affinity
|
VIII Select
|
11
|
0.046
|
382.0
|
8304
|
4.20
|
78%
|
36%
|
|
4 + 5
|
Anion exchange + size exclusion
|
Capto Q + Superdex 75
|
10
|
0.026
|
284.1
|
10925
|
2.84
|
68%
|
24%
|
-
a1–3 protein by Bradford; stage 4 + 5 by OD280 nm
