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Table 2 FVIII purification sheet

From: Stable high-level expression of factor VIII in Chinese hamster ovary cells in improved elongation factor-1 alpha-based system

# Name Resin Volume, ml Protein, mg/mla FVIII:C, IU/ml Specific activity, IU/mg Total FVIII:C, kIU Stage yield Overall yield
0 Clarified medium - 320 -- 36.3 - 11.6 - -
1 Multi-mode anion exchange Capto MMC 17 0.233 496.9 2133 8.45 73% 73%
2 Cation exchange SP Sepharose 10 0.271 538.1 1986 5.38 64% 46%
3 Affinity VIII Select 11 0.046 382.0 8304 4.20 78% 36%
4 + 5 Anion exchange + size exclusion Capto Q + Superdex 75 10 0.026 284.1 10925 2.84 68% 24%
  1. a1–3 protein by Bradford; stage 4 + 5 by OD280 nm