Fig. 6

Induction of oxidative stress by the sodium butyrate and its prevention by the BHA or fetal bovine serum increases volumetric productivity of the 11A4H cell line in batch culture. Panels a, c – batch cultivation in the presence of sodium butyrate, 72 h. Panels b, d – batch cultivation in the presence of 0.5 mM sodium butyrate (But), 10 μM BHA (BHA), 10% FBS (FBS), 0.1 mM H₂O₂ (H₂O₂), 96 h. Panels c, d – flow cytometry analysis of DCF-stained cells, samples match those on panels a, b. Cells were seeded at 3.75 × 10⁵ cells/ml. Data are presented as mean ± standard deviations, n = 2–3. The statistical significance of the difference between groups was calculated by the unpaired t-test (* - P < 0.05; ** - P < 0.01). The difference was assessed between the “intact” or “0 mM” group and other groups, if not indicated by brackets