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Table 1 Apparent k cat values and yields of n-pentadecane of WT 1593, WT sll0208 and variants. The apparent k cat values were determined using 2 mM n-heptanal as the substrate

From: Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship

   k cat app (Min−1) Yield of n-pentadecane (μM)
1593 WT 0.45 ± 0.06 12.1 ± 0.4
L146T 0.87 ± 0.1 14.2 ± 1.2
N149A 0.31 ± 0.04 8.7 ± 0.7
F150Y 0.76 ± 0.08 12.2 ± 0.6
N123H 0.43 ± 0.05 10.4 ± 0.9
Q49H/F150Y 0.72 ± 0.07 14.4 ± 0.6
Q49H/N123H/F150Y 0.87 ± 0.09 15.0 ± 0.1
Y39F 0.37 ± 0.05 10.4 ± 0.8
Q110L 0.23 ± 0.03 5.8 ± 0.2
Y122F 0.15 ± 0.02 2.1 ± 0.04
R62A 0.04 ± 0.001 0.4 ± 0.06
D143A 0.36 ± 0.05 9.2 ± 0.2
W178R 1.47 ± 0.1 17.4 ± 0.2
sll0208 WT 0.44 ± 0.05 1.4 ± 0.1
T146L 0.17 ± 0.02 1.4 ± 0.1
L148R 0.75 ± 0.08 5.1 ± 0.2
Y150F 0.42 ± 0.04 2.2 ± 0.1
D49H 0.22 ± 0.03 1.8 ± 0.1
N123H 0.14 ± 0.02 1.0 ± 0.08
D49H/N123H 0.73 ± 0.08 3.4 ± 0.3
  1. The yield of n-pentadecane was determined using 150 μM using n-hxadecanal as the substrate