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Table 1 Apparent k cat values and yields of n-pentadecane of WT 1593, WT sll0208 and variants. The apparent k cat values were determined using 2 mM n-heptanal as the substrate

From: Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship

  

k cat app (Min−1)

Yield of n-pentadecane (μM)

1593

WT

0.45 ± 0.06

12.1 ± 0.4

L146T

0.87 ± 0.1

14.2 ± 1.2

N149A

0.31 ± 0.04

8.7 ± 0.7

F150Y

0.76 ± 0.08

12.2 ± 0.6

N123H

0.43 ± 0.05

10.4 ± 0.9

Q49H/F150Y

0.72 ± 0.07

14.4 ± 0.6

Q49H/N123H/F150Y

0.87 ± 0.09

15.0 ± 0.1

Y39F

0.37 ± 0.05

10.4 ± 0.8

Q110L

0.23 ± 0.03

5.8 ± 0.2

Y122F

0.15 ± 0.02

2.1 ± 0.04

R62A

0.04 ± 0.001

0.4 ± 0.06

D143A

0.36 ± 0.05

9.2 ± 0.2

W178R

1.47 ± 0.1

17.4 ± 0.2

sll0208

WT

0.44 ± 0.05

1.4 ± 0.1

T146L

0.17 ± 0.02

1.4 ± 0.1

L148R

0.75 ± 0.08

5.1 ± 0.2

Y150F

0.42 ± 0.04

2.2 ± 0.1

D49H

0.22 ± 0.03

1.8 ± 0.1

N123H

0.14 ± 0.02

1.0 ± 0.08

D49H/N123H

0.73 ± 0.08

3.4 ± 0.3

  1. The yield of n-pentadecane was determined using 150 μM using n-hxadecanal as the substrate
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BMC Biotechnology

ISSN: 1472-6750

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