Fig. 2
Analysis of the expression level of CNA (a, b and c) and interaction of CNA with CaM (d) under Al stress. qRT-PCR analysis of the transcription levels of CNA in the presence of different concentrations of Al (a) and different treatment time under 50 mM Al3+ (b). The results were expressed as relative values with respect to 0 mM or 0 h, which were set to 1.0, respectively. The data are presented as the mean ± SE (n = 3). c Expression analysis of CNA under Al stress. Total protein was extracted from cells treated with 5, 20, 50, 100, or 150 mM Al3+. The membranes were probed with mouse antibodies against CNA and with peroxidase-conjugated goat antibodies against mouse IgG. d The interaction level of CNA with CaM under Al stress. Purified GST-CaM and total protein from cells treated by Al were incubated together. GST agarose resin was added. The interaction was detected with anti-CNA and a specific polyclonal anti-CaM antibody