Table 4 Substrate specificity of recombinant AmyA1
Substrate (5 g/L) | Main linkage/monomer | Relative activity (%)a |
|---|---|---|
Soluble starch | α- (1 → 4)-α- (1 → 6) glucose | 100 ± 3.1 |
Maize starch | α- (1 → 4)-α- (1 → 6) glucose | 74.1 ± 4.2 |
Potato starch | α- (1 → 4)-α- (1 → 6) glucose | 81.3 ± 2.8 |
Amylose | α- (1 → 4) glucose | 54.1 ± 1.9 |
Amylopectin | α- (1 → 4)-α- (1 → 6) glucose | 69.2 ± 3.5 |
Glycogen | α- (1 → 4)-α- (1 → 6) glucose | 46.1 ± 2.6 |
Pullulan | α- (1 → 6) glucose | 8.2 ± 1.5 |
maltohexaose | α- (1 → 4) glucose | 38.9 ± 3.8 |
maltopentaose | α- (1 → 4) glucose | 30.5 ± 3.5 |
maltotetraose | α- (1 → 4) glucose | 7.7 ± 1.7 |
maltotriose | α- (1 → 4) glucose | 6.1 ± 1.3 |