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Table 2 Kinetic parameters of α-L-rhamnosidase with and without sorbitol

From: Enhancing the thermostability of α-L-rhamnosidase from Aspergillus terreus and the enzymatic conversion of rutin to isoquercitrin by adding sorbitol

Sample

Specific activityA

(U/mg)

K M A

(mM)

k cat A

(s−1)

k cat /K M

(s −1M -1)

No additives

451.5 ± 12.5

0.481 ± 0.01

407.6 ± 11.6

8.5 × 103

0.6 M Sorbitol

414.5 ± 11.6

0.493 ± 0.02

379.2 ± 10.6

7.7 × 103

1.0 M Sorbitol

354.4 ± 10.4

0.542 ± 0.02

362.5 ± 10.7

6.7 × 103

  1. AValues are the means ± SD (n = 3)
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BMC Biotechnology

ISSN: 1472-6750

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