Table 1 Reagent cost analysis
Reagent | Cost | Source |
|---|---|---|
EZ-Tet-pLKO plasmid | $65 | Addgene |
PEG-8000 | $38 (per 250 g) | Sigma |
shRNA oligo (sense/antisense) | $22 (ea.) | IDT |
Screening primer (Fwd/Rev) | $8 (ea.) | IDT |
Cloning enzymes (PNK, AP, ligase) | $2.50 (per ligation) | NEB, Affymetrix |
Restriction enzymes | $0.25 (per digest) | NEB |
PCR reagents | $0.25 (per reaction) | Empirical Bioscience |
Total cost to clone first shRNA | ~$170 | |
Cost per subsequent shRNA | ~$50 |