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Fig. 1 | BMC Biotechnology

Fig. 1

From: Constitutive expression of active microbial transglutaminase in Escherichia coli and comparative characterization to a known variant

Fig. 1

Synthetic mTG construct used for transformation of E. coli BL21 (DE3) cells. The construct consists of restriction enzyme sites, the pro-domain required for folding and the mTG gene separately expressed. Each one has a preceding PelB sequence to direct periplasm secretion, whereupon folding will occur in the periplasm. Another ribosome binding site (RBS) precedes the mTG gene to ensure proper translation

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BMC Biotechnology

ISSN: 1472-6750

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