Fig. 2

Antibody concentrations of different stable cell pools. a Antibody expression levels from generated pools were measured by ELISA. The error bars represent standard deviation of triplicate ELISA measurements. Data was statistically analyzed using ANOVA to detect significant differences between the generated cell pools (p < 0.05). b Western blot analysis of reduced supernatants from four pools using HRP conjugated goat anti-human IgG. The appearance of bands with the expected size of 50 KD for heavy chain and 25 KD for light chain verified the presence of antibody. Negative control (untransfected cell culture medium) (lane 1), Positive control (human IgG) (lane 2), protein molecular weight marker (lane 3), sample supernatants of CHO-HL (lane 4), CHO-HUL (lane 5), CHO-UHUL (lane 6) and CHO-UHL (lane 7)