Fig. 3
From: Automated freeze-thaw cycles for decellularization of tendon tissue - a pilot study
Histological assessment of decellularization effectiveness. Representative images of hematoxylin and eosin stained equine superficial digital flexor tendon samples of group 1 (Auto-Protocol 1) (a), group 2 (Auto-Protocol 2) (b), group 3 (Manual Protocol) (c), showing an apparent reduction of visible nuclei compared with tendon samples of the internal controls (no decellularization) (d). Decellularized tendon samples of all groups reveal regularly aligned collagen fibrils and interfibrillar tissue gaps instead of resident cells