Fig. 5

Sequencing results for Human genomic DNA. Libraries are prepared using 50 ng of input DNA. Similar enzyme concentrations of Tn5-059 and standard Tn5 are used and 2x151 bp sequencing run is performed on a HiSeqX. a Uniformity of Coverage. Sequencing results are down sampled to 20× coverage. Tn5-059 shows improved uniformity over standard Tn5 in NexteraV2 kit. b Normalized GC plot. Grey bar shows schematic GC composition of Human genome. Standard Tn5 has a clear bias towards GC rich regions while undercovers AT rich regions. c AT/GC dropout. Tn5-059 improves AT dropout while adding little on GC dropout