Table 1 Summary of success frequencies in expression of 40 recombinant proteins in E. coli using two expression vectors, pCPD and pMCSG53^a

Total target proteins

40

40

40

Expressed proteins in E. coli

28

(70%)

18

(45%)

30

(75%)

Soluble proteins

24

(60%)

13

(33%)

26

(65%)

Purified recombinant proteins

20

(50%)

Soluble cleaved proteins

16

(40%)

21^b

(53%)