Fig. 7
Protein size analysis of refolded and soluble MnP. a Dynamic light scattering results of refolded MnP. The percentage of the protein mass (%) was plotted against the hydrodynamic radius (nm). Size exclusion chromatography of refolded MnP purified from Ni-NTA affinity chromatography was shown in the inset. Protein samples were collected after elution with 120 and 200Â mM imidazole using 5-ml Ni Sepharoseâ„¢ 6 Fast Flow. Elution profile of protein was plotted against the absorbance at 280Â nm. b & c Dynamic light scattering results of soluble MnP. The percentage of the protein mass (%) was plotted against the hydrodynamic radius (nm). Size exclusion chromatography of soluble MnP from Ni-NTA affinity chromatography was shown in the inset