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Fig. 7 | BMC Biotechnology

Fig. 7

From: Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes

Fig. 7

Protein size analysis of refolded and soluble MnP. a Dynamic light scattering results of refolded MnP. The percentage of the protein mass (%) was plotted against the hydrodynamic radius (nm). Size exclusion chromatography of refolded MnP purified from Ni-NTA affinity chromatography was shown in the inset. Protein samples were collected after elution with 120 and 200 mM imidazole using 5-ml Ni Sepharose™ 6 Fast Flow. Elution profile of protein was plotted against the absorbance at 280 nm. b & c Dynamic light scattering results of soluble MnP. The percentage of the protein mass (%) was plotted against the hydrodynamic radius (nm). Size exclusion chromatography of soluble MnP from Ni-NTA affinity chromatography was shown in the inset

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