Table 3 Oligonucleotides purification and source used to assembly the gene C
Oligonucleotide source | Supplier | Purification | Number of clones sequenced | Clones without errors | Number of bases deleted, inserted or substituted | Number of deletions | Number of insertions | Number of substitutions |
|---|---|---|---|---|---|---|---|---|
A1 | A | desalted | 16 | 68% | 6 | 4 | 1 | 1 |
B1 | B | desalted | 16 | 80% | 3 | 2 | 0 | 1 |
C1 | C | desalted | 16 | 71% | 7 | 3 | 2 | 2 |
C2 | C | cartridge | 16 | 50% | 14 | 4 | 5 | 5 |
C3 | C | HPLC | 16 | 56% | 11 | 5 | 4 | 2 |
Total | 80 | 41 | 18 (18/41 = 44%) | 12 (12/41 = 29%) | 11 (11/41 = 27%) |