Table 2 Gene synthesis error rates when were used different DNA polymerases
DNA polymerase | Hot Start activity | Number of clones sequenced | Bases sequenced | Number of bases deleted, inserted or substituted | Error rate (error/kb) | Number of deletions | Number of insertions | Number of substitutions |
|---|---|---|---|---|---|---|---|---|
KOD HS DNA pol. | yes | 24 | 5720 | 5 | 0.87 | 2 | 1 | 2 |
Q5® HS HF DNA pol. | yes | 24 | 6240 | 10 | 1.6 | 6 | 1 | 3 |
NZYProof DNA pol. | no | 24 | 6240 | 10 | 1.6 | 5 | 2 | 3 |
NZYTaq DNA pol. | no | 24 | 5980 | 13 | 2.2 | 2 | 3 | 7 |
Total | 96 | 24,180 | 38 | 15 | 7 | 15 |