Table 1 Gene assembly strategies using for production of the synthetic gene A
Gene assembly method | DNA template | Number of primers | Primer size (nt) | Cloning method |
|---|---|---|---|---|
PCA-DT_A1 | yes | 2 | 135 | Homologous recombination |
PCA-DT_A2 | yes | 13 | 27–40 | Homologous recombination |
PCA-DT_A3 | yes | 12 | 35–40 | Homologous recombination |
PCA-DTF_B1 | no | 14 | 35–40 | Gateway system |
PCA-DTF_B2 | no | 12 | 30–40 | Gateway system |
PCA-DTF_B3 | no | 8 | 35–60 | Gateway system |