Fig. 7

Agarose gel electrophoresis of 96 nucleic acids encoding venom peptides assembled simultaneously using a large scale gene synthesis platform. 98% of gene products presented high yield and correct size. 2 out the 96 genes (3 and 52) showed PCR fragments with low yield. However, the 96 PCR products were used for subsequent cloning reaction and resulting plasmids were employed to transform E. coli cells. Resulting recombinant plasmids were purified and the integrity of DNA sequences was verified by Sanger sequencing