Fig. 4

Influence of oligonucleotide concentration in the efficacy of the assembly reaction. a: PCR assembly was performed with 200, 600 and 1000 nM of outer oligonucleotides using 0.2 or 0.4 mM dNTPs and 10 nM or 30 nM of inner primers. A control reaction containing no primers was performed under same conditions (outer primers concentration:1000 nM and 0.4 mM of dNTPs). b: Outer primers concentration was fixed to 800 nM and inner primer concentration varied between 10 to 30 nM. Lanes 1–5 correspond to: 0.1, 0.2, 0.3, 0.4 and 0.5 mM dNTPs. M1: NZYLadder III; M2: NZYLadder I