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Table 3 Design of experiment for enzymatic hydrolysis of pretreated rice straw and the associated response data

From: Biochemical characterization and synergism of cellulolytic enzyme system from Chaetomium globosum on rice straw saccharification

Run no.

% composition

Reducing sugar

(mg/g biomass)

BCC5776

Novozyme®188

Accellerase®XY

Average

SD

1

100.0

0.0

0.0

500.9

2.6

2

66.7

33.3

0.0

493.1

2.2

3

66.7

0.0

33.3

525.4

0.5

4

33.3

66.7

0.0

421.2

4.3

5

33.3

33.3

33.3

535.6

2.6

6

33.3

0.0

66.7

519.3

3.2

7

0.0

100.0

0.0

69.2

3.4

8

0.0

66.7

33.3

205.3

4.1

9

0.0

33.3

66.7

233.3

4.6

10

0.0

0.0

100.0

205.0

4.9

11

66.7

16.7

16.7

567.8

1.7

12

16.7

66.7

16.7

426.1

1.4

13

16.7

16.7

66.7

483.5

2.1

  1. Reactions (1 mL) contained 5% (w/v) alkaline-pretreated rice straw in 100 mM sodium acetate buffer, pH 5.5 with a total enzyme volume of 80 μL and incubated at 50 °C for 48 h