Fig. 4

Saccharification of pretreated rice straw using the optimal enzyme mixture compared with individual enzymes. The reactions (1 mL) contained 5% (w/v) alkaline-pretreated rice straw in 100 mM sodium acetate buffer, pH 5.5 with a fixed total enzyme volume of 80 μL (1×) and incubated at 50 °C for 48 h. (a) sugar profile of optimal enzyme mixture; (b) the total reducing sugars (bar) and individual sugar profile ((■) glucose; (▲) xylose; (♦) arabinose) versus FPU loading/g on pretreated rice straw hydrolysis. *1× enzyme loading equaled to 1.49 FPU/g