Fig. 2

Effects of pH and temperature on the activity and stability of the recombinant Xyn11A-LC and mutants. a Effect of pH on the activity of Xyn11A-LC and mutants. The assay was performed in different pH buffer ranging from pH 4.5 to pH 10.0 at optimal temperature for 10 min. b Effect of pH on the stability of Xyn11A-LC and mutants. The enzyme was diluted in different buffer (pH 4.5-pH 10.0) at 37 °C for an hour, and the residual activities were measured at the optimal condition for 10 min. The activity of the purified enzyme without pre-incubation was set as 100 %. c Effect of temperature on the activity of Xyn11A-LC and mutants. The assay was performed at different temperature ranging from 30 to 70 °C for 10 min. d Effect of temperature on the stability of Xyn11A-LC and mutants. The purified enzymes were incubated in 50 mM Tris–HCl buffer (pH 8.0) without substrate for 30 min at 60 °C, respectively and taken out every 5 min. The residual xylanase activities were measured under the optimal condition for 10 min. The 0 min value was set as 100 %