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Fig. 3 | BMC Biotechnology

Fig. 3

From: Novel prokaryotic expression of thioredoxin-fused insulinoma associated protein tyrosine phosphatase 2 (IA-2), its characterization and immunodiagnostic application

Fig. 3

Purification of TrxIA-2icby affinity chromatography. Analysis of TrxIA-2ic fractions at different stages of purification by a: SDS-PAGE (10.0 % T 6.0 % C, 1 mm thickness, under reducing conditions, stained with Coomassie Brilliant Blue R-250) and b: WB revealed with a rabbit polyclonal serum to thioredoxin as primary antibody. In A and B, lane 1 corresponds to total ISF from transformed E. coli GI724, lane 2: unbound material or pass through of the column, lanes 3-5: washes with increasing 2ME concentrations (0, 1 and 5 mM), lanes 6-9: 100 mM consecutive eluates of purified TrxIA-2ic. Arrows indicate the electrophoretic mobility of TrxIA-2ic

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BMC Biotechnology

ISSN: 1472-6750

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