Table 6 Oligos used for cloning and plasmid construction

Pcmv top-PmeI

atcatgaccggtttaaacCGTTACATAACTTACGGTAA

Pcmv top-BglII

atcaccggtgctagcagatctcgcgaCGTTACATAACTTACGGTAA

Tsv40 bot-BglII

atccctaggctgcagatctGCAGTGAAAAAAATGCTTTA

Tsv40 bot-PmeI

agctgtacagatatcgagctcgtttaaacGCAGTGAAAAAAATGCTTTA

Hyg top-HindIII

atcctgaagcttacatgtccATGGGTAAAAAGCCTGAACTCACCGCGACG

Hyg bot-NheI-ClaI

atcgattagctagcTACTTCTACACAGCCATCGGTCC

Tscleu2 top-ClaI-PstI

atcgattagctgcagtAATCCTTGCTTAAAAAGATTCTCT

Tscleu2 bot-PmeI

agctgtacagatatcgagctcgtttaAACTCCATCAAATGGTCAGG

HSVtk top-SpeI-NcoI-HIndIII

atcaagcttccatggaaactagtGCGTTCGACCAGGCTGCGCGTT

HSVtk bot-XmaI-NcoI

atcgattaacccatggccCGGGCAAACGTGCGCG

Padh1 top-PmeI

tcaggcccttgaattcgtttaaacCCTACAACAACTAAGAAAATGGCTATCATGCGGAAG

Padh1 bot-HindIII-NheI

atcgctagcAAgcTTCTCTTGCTTAAAGAAAAGAAAAGCGAAGGCACCTGTCCACCACCC

100 bp-bot

GAATTCGAGCTCGTTTAAAC

Tscadh1 For-AscI

tgaggCGCGCCACTTCTAAATAAGCG

Tscadh1 R110-BglII

aagatctCCTAGCGGATCTGCCGGTAG

Amp rev

CGACACGGAAATGTTGAATACTC

ori-AflIII

CCTTTTGCTGGCCTTTTGCTCACAT

lox71-Padh1 top-BglII

acggttaattaagatctaccgttcgtatagcatacattatacgaagttatactagtcatatgCCTACAACAACTAAGAAAATGGCTATCA

lox66-Ttef bot-PmeI

atcgaattcgagctcgtttaaactaccgttcgtataatgtatgctatacgaagttataggcctaGGATGGCGGCGTTAGTATCG

nls-Cre top-XbaI

atttaattaactctagaTGTACTCCACCAAAGAAGAAGAGAAAGGTTGCCcctagcATGGCCAATTTACTGACCGTACACCA

Cre bot-XbaI

aggctagcggcgcgccTCAtctagaATCGCCATCTTCCAGCAGGC

ura4 F530U-BglII

ggcatagatctgagctcgatatcAAGCTTAGCTACAAATCCCACTGGCTATATGT

ura4 R190D-PmeI

ttacctgagctcgtttaaacAAGCTTGTGATATTGACGAAACTTTTTGACATCTAAT

leu1 F130U-BglII

aagcttagatctCCATACGATATCCCAATCTGTAG

leu1 R200D-PmeI

atcgagctcgtttaaacGGATGTCGTAAATCAATTCCATGC

arg3 F130U-BglII

aagatcTCGGCTATATGCAATCTCAC

arg3 R130D-PmeI

aagtttaaacAGCCTGTGTCCTGCGCATA

ade6 F300U-BglII

aagatcTAAGGTATAACGACAACAAACGTTGC

ade6 R150D-PmeI

aagtttaaaCTGCTTCACAGCACATTATTCAGGATTCTT

aur1 F333U-BglII

aagtcagcggcgcgccaagatctGTCAGGAAAGCTTTTTTGCCTCT

aur1 R280D-PmeI

agaattcgagctcgtttaaaCAAATACCTATACATCACACTGGAA

3xHA top

atccccgggttaattaacgctagcatgTACCCATACGATGTTCCTGACTATGCGGGCTACCCGTATGACGTGCCGGACTATGCAGGAGCCTATCCTTATGACGTTCCAGA

3xHA bot

aggctagcggcgcgccTCAtctagaACCAGCGTAATCTGGAACGTCATAAGGATAGGCTCCTGCATAGTCCGGCACGTCATACGGGTAGCCCGCATAGTCAGGAACATCG

3xPK-H6 top

ggatccatatgTTAATTAACGCTAGCGGTAAGCCTATTCCTAACCCTCTTTTGGGTCTCGATTCTACAGGTGGAAAACCAATCCCCAACCCACTCCTCGGCCTTGACTCAACTGGCAAAC

3xPK-H6 bot

agatctggcgcgcctaggatggtgatggtgatgatgtctagaGGTACTATCTAATCCAAGTAAAGGATTTGGTATGGGTTTGCCAGTTGAGTCAAGGCCGAGGAGTGGGTTGGGGATTGG

3xFlag top

taacgctagcatgGGTGACTACAAGGACGACGATGACAAGGGAGATTACAAAGATGACGACGATAAGGACTACAAGGACGACGATGACAAGtctagaTGAgg

3xFlag bot

cgcgccTCAtctagaCTTGTCATCGTCGTCCTTGTAGTCCTTATCGTCGTCATCTTTGTAATCTCCCTTGTCATCGTCGTCCTTGTAGTCACCcatgctagcgttaat

tpz1 F800U-PvuII-EcoO

atcaggccctcagCTGTTCAGCACGGTACCAAG

tpz1 R1745-NheI

gttgcagctagcGCTTTTGTTTCGAAACTCCTCTAT