Sequential and counter-selectable cassettes for fission yeast

BMC Biotechnology

Table 1 One hundred base primers for PCR-based targeting fragments used in this study

Name	Sequence
crb2 Top	CCCTGGTTAAATTTGTAGTTCTGACAATTGTGAGGTATTTTAGATGTTTTCAATATTTTGTTTGAAAGTTTAACAATATTCGGATCCCCGGGTTAATTAA
crb2 Bot	CTAAAATTAATAAAAAGCTAAATTAATGAGAGTGAAACTCAGGGGGAGTTAGTAAAAATAACTATATCAAAAAACCAAAAGAATTCGAGCTCGTTTAAAC
rdh54 Top	ACTTGAACACCACACGTTCGGTCTCATAGTATTGTTGAGTAAATAAACACAGTTACCAAAGAGAATTGAAACCTTACTTTCGGATCCCCGGGTTAATTAA
rdh54 Bot	TGCTCATAGAGATTGCCCAGTATCGAAGGCTTGCTCAAAAGCGTTGTTTCTAATGGGCAAGGAAAAACCGCTTACGCCTCGAATTCGAGCTCGTTTAAAC
tpz1 Tag	CTCTGAGGCCTGTGAAATGTGTCGGCTTGGGCTACCTCATGGATCATTCTTTGAGCTATTGCGAGATTGGAAAAAAATAGAGGAGTTTCGAAACAAAAGCCGGATCCCCGGGTTAATTAA
tpz1 Bot	TTGGTCCGTTGTAAGCCATTTCACTGTATGTCTGTAACAGTTAACTTCCGTACTTAGTAAAATGTTAGTAAAAAAGGAAGATATGTGATACAGCAATTGAGAATTCGAGCTCGTTTAAAC
tpz1 Top	ATCAACAGACTTCAGTCAGCACTGTTACTTATTAAAAAAAGTTGATTTTTATATAAAAGTTAGCTGCGTTAAACAGTGCACGGATCCCCGGGTTAATTAA

Under line encodes annealing site for the template. Top primer contains CGGATCCCCGGGTTAATTAA at the 3’ end and 80–100 base upstream sequence of the target site at the 5’ end. Bot primer contains GAATTCGAGCTCGTTTAAAC at the 3’ end and 80–100 base downstream complement sequence of the target site at the 5’ end

ISSN: 1472-6750