Fig. 5

Cre expression vector and loxP cassette. a Schematic representation of loxP-flanked (floxed) TK-fusion cassettes FHyTKAX, FTKanAX and FTKnatAX. Region used as a PCR template is shown. Forward direction of lox71 and lox66 sequences are inserted into the TK cassettes shown in Fig. 2a. b Schematic representation of pNXRVa-HACre. Fusion protein of three tandem HA epitope tag, NLS (nuclear localization signal) and Cre recombinase is expressed by the constitutively active CMV promoter. kanMX6 is inserted between BglII and PmeI sites. The genomic DNA fragment containing the early replication origin ARS1 is inserted downstream of the kanMX6 cassette. A number of unique restriction enzyme sites are indicated