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Table 1 Apparent kinetic parameters of BgADH3a

From: Efficient biosynthesis of ethyl (R)-4-chloro-3-hydroxybutyrate using a stereoselective carbonyl reductase from Burkholderia gladioli

Substrate

NADH

NADPH

K m (mM)

0.058

0.081

V max (μmol min−1 mg−1)

3.4

9.1

k cat (s−1)

3.97

10.6

k cat/K m (mM−1s−1)

68.3

131

  1. aReaction conditions: substrate COBE (50 mM), NADH or NADPH (0.01–0.5 mM), purified BgADH3 (0.1 mg mL−1), pH 6.5, and 40 °C
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BMC Biotechnology

ISSN: 1472-6750

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