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Fig. 3 | BMC Biotechnology

Fig. 3

From: A novel set of vectors for Fur-controlled protein expression under iron deprivation in Escherichia coli

Fig. 3

Vector map of pFBH1. a Polypeptide sequence immediately downstream of the bidirectional promoter region found in gBlock3. Start codon sequence: black, HA sequence: red, TEV cleavage sequence: blue. MCS region from pUT18C colored by codons with restriction endonuclease sites shown below sequence. b pFBH1 vector map. Light blue bar indicates the promoter region. Unique restriction endonuclease sites shown in bold. HA: HA tag sequence; TEV: TEV cleavage site, AmpR: ampicillin resistance gene; ori: pMB1 origin of replication

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