Fig. 2
Expression of GBP-SubA. a. E. coli Rosetta (DE3) containing pET-28a or pET-28a-GBP-SubA was induced by 1 mM IPTG at 37 °C for 4 h, and then lysed with ultrasound. Lane 1, 3 and 5: the total cellular lysate, soluble lysate fraction and insoluble lysate fraction from E. coli Rosetta (DE3) containing pET-28a plasmid, respectively; lane 2, 4 and 6: total cellular lysate, soluble lysate fraction and insoluble lysate fraction from E. coli Rosetta (DE3) containing pET-28a-GBP-SubA plasmid, respectively; Lane M: marker. b. Induction of GBP-SubA with different concentrations of IPTG at 37 °C. lane 1–3: total cellular lysate, soluble lysate fraction and insoluble lysate fraction of E. coli Rosetta (DE3) containing pET-28a-GBP-SubA plasmid that induced by 0.1 mM IPTG; lane 4–6: total cellular lysate, soluble lysate fraction and insoluble lysate fraction of E. coli Rosetta (DE3) containing pET-28a-GBP-SubA plasmid that induced by 0.5 mM IPTG; lane 7–9: total cellular lysate, soluble lysate fraction and insoluble lysate fraction of E. coli Rosetta (DE3) containing pET-28a-GBP-SubA plasmid that induced by 1 mM IPTG; lane 10–12: total cellular lysate, soluble lysate fraction and insoluble lysate fraction of E. coli Rosetta (DE3) containing pET-28a-GBP-SubA plasmid that induced by 2 mM IPTG; Lane M: marker. c. Induction of GBP-SubA with different concentrations of IPTG at 16 °C. Lane 1–2: soluble lysate fraction and insoluble lysate fraction of E. coli Rosetta (DE3) containing pET-28a-GBP-SubA plasmid that induced by 0.1 mM IPTG; lane 3–4: soluble lysate fraction and insoluble lysate fraction of E. coli Rosetta (DE3) containing pET-28a-GBP-SubA plasmid that induced by 0.5 mM IPTG; lane 5–6: soluble lysate fraction and insoluble lysate fraction of E. coli Rosetta (DE3) containing pET-28a-GBP-SubA plasmid that induced by 1 mM IPTG; lane 7–8: soluble lysate fraction and insoluble lysate fraction of E. coli Rosetta (DE3) containing pET-28a-GBP-SubA plasmid that induced by 2 mM IPTG; Lane M: marker