Table 2 Primers used in this study
From: High-specificity synthesis of novel monomers by remodeled alcohol hydroxylase
Name | Sequence (5′ → 3′) |
|---|---|
BM3-NcoF | CTTGCCATGGGCATGACAATTAAAGAAATGCCTCAG |
BM3-BamR | CGGGATCCTTACCCAGCCCACACGTCTTTTG |
BM3J-NcoF | CATGCCATGGGCATGACAATTAAAGAAATGCCTCAG |
BM3J-EcoR | GACGGAATTCTTCCACATCG |
BM3J-EcoF | GAAGAATTCCGTCCAGAGCGTTTTG |
BM3J-NotR | ATAAGAATGCGGCCGCTTACCCAGCCCACACGTCTTTTG |
BM3-V78A-F | GCTTAAATTTGCACGTGATTTTGCAGGAGACGG |
BM3-V78A-R | CAAAATCACGTGCAAATTTAAGCGCTTGACTTAAG |
BM3-T175I-F | CCATTTATTATCAGTATGGTCCGTGCACTGGATG |
BM3-T175I-R | GACCATACTGATAATAAATGGATGAGGCTGATC |
BM3-A184V-F | GGATGAAGTAATGAACAAGCTGCAGCGAGC |
BM3-A184V-R | CTTGTTCATTACTTCATCCAGTGCACGGACC |
BM3-F205C-F | CAAGCGCCAGTGTCAAGAAGATATCAAGGTG |
BM3-F205C-R | CTTCTTGACACTGGCGCTTGTTTTCATCATAAG |
BM3-S226R-F | CGCAAAGCACGCGGTGAACAAAGCGATG |
BM3-S226R-R | GTTCACCGCGTGCTTTGCGATCTGC |
BM3-H236Q-F | CGCAGATGCTAAACGGAAAAGATCCAG |
BM3-H236Q-R | CCGTTTAGCATCTGCGTTAATAAATCATC |
BM3-E252G-F | GATGACGGGAACATTCGCTATCAAATTATTAC |
BM3-E252G-R | GCGAATGTTCCCGTCATCAAGCGGCTCACCCG |
BM3-R255S-F | GACGAGAACATTAGCTATCAAATTATTACATTC |
BM3-R255S-R | GATAGCTAATGTTCTCGTCATCAAGCGGCTCAC |
BM3-A290V-F | GTATTACAAAAAGTAGCAGAAGAAGCAGC |
BM3-A290V-R | CTTCTGCTACTTTTTGTAATACATGTGG |
BM3-L353V-F | GCGACGAAGTAATGGTTCTGATTCCTCAGC |
BM3-L353V-R | GAACCATTACTTCGTCGCCTTTTTCTAAAGG |
BM3J-A78F-F | GCGCTGAAATTTTTCCGTGATTTTGCAGGTGACGG |
BM3J-A78F-R | CAAAATCACGGAAAAATTTCAGCGCTTGACTTAAG |
J2-R47L-F | GCGCCTGGTCTGGTAACGCGCTACTTATCAAG |
J2-R47L-R | CGCGTTACCAGACCAGGCGCCTCGAATTTAAAG |
J2-F87A-F | GACGGGTTGGCTACAAGCTGGACGCATG |
J2-F87A-R | GCTTGTAGCCAACCCGTCACCTGCAAAATC |