Fig. 4

Effects of various divalent metal ions at 0.5 mM (a) and Mn²⁺ concentration (b) on BCAI activity. Enzyme assay was carried out at 60 °C and pH 7.5. None: purified BCAI without EDTA treatment; EDTA: purified BCAI treated by EDTA; The EDTA treated enzyme was used when determining of the effects of metal ions and Mn²⁺ concentration. Mn²⁺ + Co²⁺: the mixture of 0.5 mM Mn²⁺ and 0.5 mM Co²⁺