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Fig. 4 | BMC Biotechnology

Fig. 4

From: Virus-like particles derived from Pichia pastoris-expressed dengue virus type 1 glycoprotein elicit homotypic virus-neutralizing envelope domain III-directed antibodies

Fig. 4

Characterization of antibodies generated by DENV-1 E VLPs. Pooled sera from mice immunized with DENV-1 E VLPs, collected on day 37 (dashed line) and day 100 (solid line) were analysed in indirect ELISA format using DENV-1 E VLPs as the coating antigen (a). Pooled day 100 immune serum was evaluated for reactivity using DENV-E VLPs (b), recombinant EDIIIs (c), and intact DENVs (d) taken as coating antigens, corresponding to serotypes 1 (red curve), 2 (green curve), 3 (blue curve) and 4 (black curve). Grey lines (in all panels) represented serum from PBS-immunized BALB/c mice. e Indirect immunofluorescence analysis of DENV-1 infected BHK-21 cells using (i) PBS-immunized serum (ii) 4G2 mAb (iii) anti-DENV-1 E VLP antiserum taken as the source of primary antibodies. Anti-mouse IgG-FITC conjugate was used to visualize bound antibodies

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BMC Biotechnology

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