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Fig. 2 | BMC Biotechnology

Fig. 2

From: Intracellular delivery of messenger RNA by recombinant PP7 virus-like particles carrying low molecular weight protamine

Fig. 2

Expression of recombinant 2PP7-Protamine-GFP VLPs in E. coli. a Expression of 2PP7-Protamine and 2PP7-Protamine-GFP VLPs in the supernatant of BL21 (DE3) after sonication. Lanes of BL21 (DE3) carrying each plasmid are designated as follows: lane 1, plasmid pETDuet-2PP7-Protamine-GFP with IPTG added; lane 2, plasmid pETDuet-2PP7-Protamine without IPTG added; lane 3, pETDuet-2PP7-Protamine without IPTG added; lane 4, pETDuet-2PP7-Protamine with IPTG added; lane 5, pETDuet-2PP7 with IPTG added. b-d Purification of the VLPs. The peaks of the target proteins are marked by red arrows

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BMC Biotechnology

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