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Table 3 H+/O ratio and ubiquinol oxidase activity of recombinant G. oxydans strains

From: Combinatorial metabolic engineering of industrial Gluconobacter oxydans DSM2343 for boosting 5-keto-D-gluconic acid accumulation

Strains

H+/O ratioa

(No. of experiments)

Ubiquinol oxidase activity

(μmol/min · mg)

5-KGA production

(g/L)

G. oxydans ZJU3

1.21 ± 0.11 (8)

0.31 ± 0.04

122.48 ± 0.41

G. oxydans ZJU4

1.19 ± 0.10 (8)

0.32 ± 0.05

131.76 ± 1.89

G. oxydans ZJU5

1.20 ± 0.11 (8)

0.30 ± 0.11

134.88 ± 2.16

G. oxydans ZJU6

1.95 ± 0.23 (8)

0.78 ± 0.05

141.86 ± 2.89

G. oxydans ZJU7

2.01 ± 0.16 (8)

0.80 ± 0.06

144.52 ± 2.94

  1. athe H+/O measured by the oxygen pulse method